production of recombinant proline dehydrogenase enzyme from pseudomonas fluorescens pf-5 in e. coli system

Production of Recombinant Proline Dehydrogenase Enzyme from Pseudomonas fluorescens pf-5 in E. coli System

Proline dehydrogenase (ProDH; 1.5.99.8) belongs to superfamily of amino acid dehydrogenase, which plays a significant role in the metabolic pathway from proline to glutamate. The goal of this research was gene cloning and characterization of ProDH enzyme from Pseudomonas fluorescens pf-5 strain. The gene encoding ProDH was isolated by means of PCR amplification and cloned in an IPTG inducible T...

Reciprocal regulation of pyoluteorin production with membrane transporter gene expression in Pseudomonas fluorescens Pf-5.

Pyoluteorin is a chlorinated polyketide antibiotic secreted by the rhizosphere bacterium Pseudomonas fluorescens Pf-5. Genes encoding enzymes and transcriptional regulators involved in pyoluteorin production are clustered in the genome of Pf-5. Sequence analysis of genes adjacent to the known pyoluteorin biosynthetic gene cluster revealed the presence of an ABC transporter system. We disrupted ...

Lon protease influences antibiotic production and UV tolerance of Pseudomonas fluorescens Pf-5.

Pseudomonas fluorescens Pf-5 is a soil bacterium that suppresses plant pathogens due in part to its production of the antibiotic pyoluteorin. Previous characterization of Pf-5 revealed three global regulators, including the stationary-phase sigma factor sigma(S) and the two-component regulators GacA and GacS, that influence both antibiotic production and stress response. In this report, we desc...

recombinant production of mecasermin in e. coli expression system

human insulin-like growth factor 1 (higf-1) consists of 70 amino acids in a single chain with three intermolecular disulfide bridges possessing valuable therapeutic effects. to date, numerous variants of specifically engineered higf-1 have been produced so as to improve higf-1 biological activity, stability and stronger binding to igf-1 receptor. mecasermin is one of the modified variants with ...

Structure of a putative BenF-like porin from Pseudomonas fluorescens Pf-5 at 2.6 A resolution.

Structure of a putative BenF-like porin from Pseudomonas fluorescens Pf-5 at 2.6 Å resolution Parthasarathy Sampathkumar,* Frances Lu, Xun Zhao, Zhenzhen Li, Jeremiah Gilmore, Kevin Bain, Marc E. Rutter, Tarun Gheyi, Kenneth D. Schwinn, Jeffrey B. Bonanno, Ursula Pieper, J. Eduardo Fajardo, Andras Fiser, Steven C. Almo, Subramanyam Swaminathan, Mark R. Chance, David Baker, Shane Atwell, Devon A...

Expression of Recombinant 3-Beta Hydroxysteroid Dehydrogenase Protein in E. coli